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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
18/02/2019 |
Data da última atualização: |
24/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TRÍBULO, P.; BALZANO-NOGUEIRA, L.; CONESA, A.; SIQUEIRA, L. G. B.; HANSEN, P. J. |
Afiliação: |
PAULA TRÍBULO, University of Florida, Gainesville, Florida; LEANDRO BALZANO-NOGUEIRA, University of Florida, Gainesville, Florida; ANA CONESA, University of Florida, Gainesville, Florida; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; PETER J. HANSEN, University of Florida, Gainesville, Florida. |
Título: |
Changes in the uterine metabolome of the cow during the first 7 days after estrus. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Molecular Reproduction and Development, v. 86, n. 1, p. 75-87, 2019. |
DOI: |
10.1002/mrd.23082 |
Idioma: |
Inglês |
Conteúdo: |
Abstract The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for the development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on Days 0 (N = 4), 3 ( N = 4), 5 ( N = 3), and 7 ( N = 4) relative to ovulation and flushed with Dulbecco's phosphate-buffered saline. A total of 1,993 molecular features were detected of which 184 peaks with putative identification represent 147 unique metabolites, including amino acids, benzoic acids, lipid molecules, carbohydrates, purines, pyrimidines, vitamins, and other intermediate and secondary metabolites. Results revealed changes in the uterine metabolome as the cow transitions from ovulation to Day 7 of the estrous cycle. The majority of metabolites that changed with day reached maximum intensity on either Day 5 or 7 relative to ovulation. Moreover, several metabolites found in the uterine fluid have signaling capabilities and some have been shown to affect preimplantation embryonic development. In conclusion, the metabolome of the bovine uterus changes during early stages of the estrous cycle and is likely to participate in the regulation of preimplantation embryonic development. Data reported here will serve as the basis for future studies aiming to evaluate maternal regulation of preimplantation embryonic development and optimal conditions for the culture of embryos. MenosAbstract The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for the development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on Days 0 (N = 4), 3 ( N = 4), 5 ( N = 3), and 7 ( N = 4) relative to ovulation and flushed with Dulbecco's phosphate-buffered saline. A total of 1,993 molecular features were detected of which 184 peaks with putative identification represent 147 unique metabolites, including amino acids, benzoic acids, lipid molecules, carbohydrates, purines, pyrimidines, vitamins, and other intermediate and secondary metabolites. Results revealed changes in the uterine metabolome as the cow transitions from ovulation to Day 7 of the estrous cycle. The majority of metabolites that changed with day reached maximum intensity on either Day 5 or 7 relative to ovulation. Moreover, several metabolites found in the uterine fluid have signaling capabilities and some have been shown to affect preimplantation embryonic development. In conclusion, the metabolome of the bovine uterus changes during early stages of the estrous cycle and is likely to participate in the regulation of preimplantation embryonic ... Mostrar Tudo |
Palavras-Chave: |
Histotroph; Maternal-embryo crosstalk. |
Thesaurus Nal: |
Endometrium; Metabolome. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02392naa a2200229 a 4500 001 2106149 005 2023-01-24 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1002/mrd.23082$2DOI 100 1 $aTRÍBULO, P. 245 $aChanges in the uterine metabolome of the cow during the first 7 days after estrus.$h[electronic resource] 260 $c2019 520 $aAbstract The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for the development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on Days 0 (N = 4), 3 ( N = 4), 5 ( N = 3), and 7 ( N = 4) relative to ovulation and flushed with Dulbecco's phosphate-buffered saline. A total of 1,993 molecular features were detected of which 184 peaks with putative identification represent 147 unique metabolites, including amino acids, benzoic acids, lipid molecules, carbohydrates, purines, pyrimidines, vitamins, and other intermediate and secondary metabolites. Results revealed changes in the uterine metabolome as the cow transitions from ovulation to Day 7 of the estrous cycle. The majority of metabolites that changed with day reached maximum intensity on either Day 5 or 7 relative to ovulation. Moreover, several metabolites found in the uterine fluid have signaling capabilities and some have been shown to affect preimplantation embryonic development. In conclusion, the metabolome of the bovine uterus changes during early stages of the estrous cycle and is likely to participate in the regulation of preimplantation embryonic development. Data reported here will serve as the basis for future studies aiming to evaluate maternal regulation of preimplantation embryonic development and optimal conditions for the culture of embryos. 650 $aEndometrium 650 $aMetabolome 653 $aHistotroph 653 $aMaternal-embryo crosstalk 700 1 $aBALZANO-NOGUEIRA, L. 700 1 $aCONESA, A. 700 1 $aSIQUEIRA, L. G. B. 700 1 $aHANSEN, P. J. 773 $tMolecular Reproduction and Development$gv. 86, n. 1, p. 75-87, 2019.
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Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
31/03/2017 |
Data da última atualização: |
13/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
OLIVEIRA, C. de; DEGENHARDT-GOLDBACH, J.; BETTENCOURT, G. M. de F.; AMANO, E.; FRANCISCON, L.; QUOIRIN, M. |
Afiliação: |
CASSIANA DE OLIVEIRA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; GISELA MANUELA DE FRANÇA BETTENCOURT; ERIKA AMANO, UFPR; LUZIANE FRANCISCON, CNPF; MARGUERITE QUOIRIN, UFPR. |
Título: |
Micropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 28, n. 1, p. 29-39, Jan. 2017. |
DOI: |
10.1007/s11676-016-0282-6 |
Idioma: |
Inglês |
Conteúdo: |
Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. MenosGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, a... Mostrar Tudo |
Palavras-Chave: |
Callogenesis; Eucalyptus urograndis; WPM culture medium. |
Thesagro: |
Micropropagação; Organogênese; Propagação vegetativa. |
Thesaurus NAL: |
Callus formation; Micropropagation; Organogenesis; Plant propagation. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02512naa a2200313 a 4500 001 2067974 005 2017-12-13 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-016-0282-6$2DOI 100 1 $aOLIVEIRA, C. de 245 $aMicropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone.$h[electronic resource] 260 $c2017 520 $aGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. 650 $aCallus formation 650 $aMicropropagation 650 $aOrganogenesis 650 $aPlant propagation 650 $aMicropropagação 650 $aOrganogênese 650 $aPropagação vegetativa 653 $aCallogenesis 653 $aEucalyptus urograndis 653 $aWPM culture medium 700 1 $aDEGENHARDT-GOLDBACH, J. 700 1 $aBETTENCOURT, G. M. de F. 700 1 $aAMANO, E. 700 1 $aFRANCISCON, L. 700 1 $aQUOIRIN, M. 773 $tJournal of Forestry Research$gv. 28, n. 1, p. 29-39, Jan. 2017.
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